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Nitric Oxide Synthase: Characterization and Functional Analysis

Autor: Mahin D. Maines, P. Michael Conn

Jazyk:

Väzba: Pevná

Vydavateľ: Elsevier Science Publishing Co Inc

Dostupnosť: U vydavateľa na objednávku

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124.54 €

Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous...

Informácie o knihe

Autor

Mahin D. Maines, P. Michael Conn

Jazyk

Angličtina

Väzba

Kniha - Pevná

Vydalo

1996

Stránok

354

EAN

9780121853013

ISBN

0121853012

Enbook ID

04493237

Vydavateľ

Elsevier Science Publishing Co Inc

Hmotnosť

910

Rozmery

191 x 235 x 24

Kompletný popis

Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field. It includes comprehensive protocols for the detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques. It contains newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression. It provides an assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone. It also includes calcium imaging by confocal microscopy, and evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and - 2 message level and distribution.

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Nitric Oxide Synthase: Characterization and Functional Analysis

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