High-Throughput RNAi Screening

Methods and Protocols

Autor: Shilpi Arora, David O. Azorsa

Jazyk:

Väzba: Brožovaná

Vydavateľ: Humana Press Inc.

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High-throughput RNAi screening remains one of the most widely used technologies to perform target id...

Informácie o knihe

Autor

Shilpi Arora, David O. Azorsa

Jazyk

Angličtina

Väzba

Kniha - Brožovaná

Vydalo

2018

Stránok

262

EAN

9781493981687

Enbook ID

20121551

Vydavateľ

Humana Press Inc.

Hmotnosť

5235

Rozmery

178 x 254 x 16

Kompletný popis

High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.

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High-Throughput RNAi Screening

Methods and Protocols

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