Fed-Batch Fermentation

Name: Fed-Batch Fermentation
Brand: Elsevier Books
SKU: 9781907568923
Price: 163.0 EUR
Availability: InStock

Autor: Garner G. Moulton

Jazyk:

Väzba: Pevná

Vydavateľ: Elsevier Books

Dostupnosť: U vydavateľa na objednávku

Odosielame za 28-34 dní

163.00 €

Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli...

Informácie o knihe

Autor

Garner G. Moulton

Jazyk

Angličtina

Väzba

Kniha - Pevná

Vydalo

2013

Stránok

188

EAN

9781907568923

ISBN

1907568921

Enbook ID

05027888

Vydavateľ

Elsevier Books

Hmotnosť

442

Rozmery

237 x 164 x 16

Kompletný popis

Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli using a fed-batch fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - fed-batch fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

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