DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols

Autor: Alan Kimmel, Brian Oliver

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Väzba: Pevná

Vydavateľ: Elsevier Science Publishing Co Inc

Dostupnosť: U vydavateľa na objednávku

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Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now...

Informácie o knihe

Autor

Alan Kimmel, Brian Oliver

Jazyk

Angličtina

Väzba

Kniha - Pevná

Vydalo

2006

Stránok

512

EAN

9780121828158

ISBN

0121828158

Enbook ID

04493221

Vydavateľ

Elsevier Science Publishing Co Inc

Hmotnosť

820

Rozmery

152 x 229 x 24

Kompletný popis

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis

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DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols

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