Developing Library-Based Bacterial Source Tracking Methods

Name: Developing Library-Based Bacterial Source Tracking Methods
Brand: Scholars' Press
SKU: 9783639708745
Price: 59.13 EUR
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Autor: Wail Hassan

Jazyk:

Väzba: Brožovaná

Vydavateľ: Scholars' Press

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The goal of this study is to develop efficient bacterial source tracking capabilities for the identi...

Informácie o knihe

Autor

Wail Hassan

Jazyk

Angličtina

Väzba

Kniha - Brožovaná

Vydalo

2014

Stránok

120

EAN

9783639708745

ISBN

3639708741

Enbook ID

07173572

Vydavateľ

Scholars' Press

Hmotnosť

186

Rozmery

152 x 229 x 7

Kompletný popis

The goal of this study is to develop efficient bacterial source tracking capabilities for the identification of sources of fecal pollution. Two polymerase chain reaction-based methods, BOX-PCR and REP-PCR, were compared to pulsed field gel electrophoresis. Additionally, source tracking fidelity was compared using two target organisms, Escherichia coli and Enterococcus spp. The highest fidelity of source tracking was achieved using BOX-PCR and Enterococcus spp. The study also showed that applying a similarity value (a measure of how similar an unknown isolate is to isolates from a known source) threshold and/or quality factor (a measure of how an unknown isolate fits within the source to which it was assigned) threshold improved the fidelity of source tracking. Finally, enterococcal isolates obtained from local rivers and from Mississippi Gulf Coast were assigned to animal sources using the method developed as part of this work. The main source of fecal contamination in river samples from the Hattiesburg area appeared to be chicken feces, while most enterococcal isolates from the Gulf Coast samples were of gull or chicken origin, with occasional human isolates.

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Developing Library-Based Bacterial Source Tracking Methods

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