The third edition of this§volume is aimed at providing both beginners and more experienced researchers a§choice of methods to isolate and analyze dendritic cells(DC). An introductory§review provides an overview of recent advances in the characterization of DC§subsets in mouse and human. While additional chapters provide methods to§culture human and mouse dendritic cells, protocols for the isolation of§dendritic cells, the isolation of dendritic cell progenitors from mouse, and§the purification of dendritic cells from human blood. Written in the highly§successful Methods in Molecular Biology series format, chapters include§introductions to their respective topics, lists of the necessary materials and§reagents, step-by-step, readily reproducible laboratory protocols, and tips on§troubleshooting and avoiding known pitfalls.§§Authoritative and§cutting-edge, Dendritic Cell§Protocols, Third Edition aims to ensure successful results in the further§study of this vital field.§