Atomic Force Microscope and Cell Biology

Name: Atomic Force Microscope and Cell Biology
Brand: VDM Verlag Dr. Müller
SKU: 9783639187205
Price: 48.34 EUR
Availability: InStock

The mechanical properties from the membrane to the interior of the cell

Autor: Charles Roduit

Jazyk:

Väzba: Brožovaná

Vydavateľ: VDM Verlag Dr. Müller

Dostupnosť: U vydavateľa na objednávku

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This book describes the doctoral thesis where we explore the mechanical properties of living cells u...

Informácie o knihe

Autor

Charles Roduit

Jazyk

Angličtina

Väzba

Kniha - Brožovaná

Vydalo

2009

Stránok

120

EAN

9783639187205

Enbook ID

06826861

Vydavateľ

VDM Verlag Dr. Müller

Hmotnosť

215

Rozmery

152 x 220 x 13

Kompletný popis

This book describes the doctoral thesis where we explore the mechanical properties of living cells using the Atomic Force Microscope (AFM). The cell membrane contains lipids microdomains, called rafts, enriched in cholesterol and sphingolipids. The lipid rafts are believed to play an important role in signal processing by acting as a signaling platform . The mechanical properties of these rafts were characterized by targeting one of its component, the GPI-anchored protein. This work revealed these domains to be stiffer than the surrounding membrane. The stiffness specificity of rafts may be related to the lower diffusion rate of proteins and can be, therefore, an important property for its role as a signaling platform. During this thesis, we also introduced a new AFM imaging mode, which we called stiffness tomography . With this imaging mode, we were able to distinguish stiff materials inclusion located into the sample. A significant part of this project was the development of a post-processing software to automate the computations of the acquired data. In this book we describes the algorithms used in the open-source version of this software.

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Atomic Force Microscope and Cell Biology

The mechanical properties from the membrane to the interior of the cell

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