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AMPK

Methods and Protocols

Autor: Dietbert Neumann, Benoit Viollet

Jazyk:

Väzba: Brožovaná

Vydavateľ: Humana Press Inc.

Dostupnosť: U vydavateľa na objednávku

Odosielame za 17-27 dní

155.38 €

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysica...

Informácie o knihe

Autor

Dietbert Neumann, Benoit Viollet

Jazyk

Angličtina

Väzba

Kniha - Brožovaná

Vydalo

2019

Stránok

626

EAN

9781493992492

Enbook ID

21295250

Vydavateľ

Humana Press Inc.

Hmotnosť

1208

Rozmery

178 x 254 x 254

Kompletný popis

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human Gamma2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.

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AMPK

Methods and Protocols

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