Membrane Lipids: Methods and Protocols

1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles

Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang

2. Preparing Ion Channel Switch Membrane-Based Biosensors

Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela

3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer

Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt

4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes

Tadas Penkauskas, Filipas Ambrulevič
ius, and Gintaras Valinč
ius

5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes

Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster

6. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)

7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield

8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

Kan Shoji

9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations

Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes

10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations

Robby Manrique

11. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins

Amanda Buyan and Ben Corry

12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling

Kathleen Wood

13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation

Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt

14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt

15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers

Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson