Host-Fungal Interactions: Methods and Protocols

1. Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans

Carol A. Woolford and Aaron P. Mitchell

2. Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens

Juliane Macheleidt and Olaf Kniemeyer

3. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification

Quynh T. Phan and Scott G. Filler

4. CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia Sara Gago, Nicola LD Overton, and Paul Bowyer

5. Analysis of Epithelial Cell Responses to Microbial Pathogens

Spyridoula Nikou, Jemima Ho, Olivia Hepworth, Nicole Ponde, Ruth Dickenson, Jonathan P. Richardson, and Julian R. Naglik

6. Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry

Margherita Bertuzzi and Gareth J. Howell

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8. Fungal Bioreporters to Monitor Outcomes of Aspergillus - Host Cell Interactions

Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, and Tobias M. Hohl

9. Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays

Selene Mogavero and Bernhard Hube

10. Experimental Evolution of Candida by Serial Passaging in Host Cells

Katja Graf, Bernhard Hube, and Sascha Brunke

11. Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells

Patawee Asamaphan, Gordon D. Brown, and Janet Willment

12. Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras

Rebecca A. Drummond and Michail S. Lionakis

13. Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole

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14. Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections

Jorge Amich

15. A Murine Model for Chronic A. fumigatus Airway Infections

Benjamin Ralph and Donald C. Sheppard

16. Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge

Sayema Rahman, Darren D. Thomson^, and Margherita Bertuzzi