Protein Sequencing and Identification Using Tandem Mass Spectrometry

The rapid expansion of searchable protein and DNA databases inrecent years has triggered an explosive growth in the applicationof mass spectrometry to protein sequencing. This timely andauthoritative book provides professionals and scientists inbiotechnology research with complete coverage of procedures foranalyzing protein sequences by mass spectrometry, includingstep-by-step guidelines for sample preparation, analysis, and datainterpretation.

Michael Kinter and Nicholas Sherman present their ownhigh-quality, laboratory-tested protocols for the analysis of awide variety of samples, demonstrating how to carry out specificexperiments and obtain fast, reliable results with a 99% successrate. Readers will get sufficient experimental detail to apply intheir own laboratories, learn about the proper selection andoperation of instruments, and gain essential insight into thefundamental principles of mass spectrometry and protein sequencing.Coverage includes:

• Peptide fragmentation and interpretation of product ionspectra
• Basic polyacrylamide gel electrophoresis
• Preparation of protein digests for sequencing experiments
• Mass spectrometric analysis using capillary liquidchromatography
• Techniques for protein identification by database searches
• Characterization of modified peptides using tandem massspectrometry

And much more