Glycolipids: Methods and Protocols

1. Immunomodulatory Functions of α
-GalCer and a Derivative, α
-Carba-GalCer

Masashi Satoh and Kazuya Iwabuchi

2. Chemical Synthesis and Molecular Interaction Analysis of a-Galactosyl Ceramide Derivatives as CD1d Ligands

Kazunari Ueki, Kodai Sueyoshi, Shinsuke Inuki, and Yukari Fujimoto

3. Immunomodulatory Functions of Glycolipids from Pathogens

Carla Guenther, Miyuki, Watanabe, and Sho Yamasaki

4. Chemical Synthesis and Immunomodulatory Functions of Bacterial Lipid As

Atsushi Shimoyama and Koichi Fukase

5. Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines

Yoshiyuki Manabe and Koichi Fukase

6. Chemical Synthesis of Phosphatidylglucoside

Koki Kano, Nozomi Ishii, Peter Greimel, and Ichiro Matsuo

7. Sialidase-Resistant Ganglioside GM3 Analogues: Evaluation of Biological Activity

Go Hirai

8. Chemical Synthesis of Gangliosides

Akihiro Imamura, Hiromune Ando, and Hideharu Ishida

9. Sialyltransferase Activity Assay for Ganglioside GM3 Synthase

Kei-ichiro Inamori, Takahiro Nitta, Fumi Shishido, Soichiro Watanabe, Isao Ohno, and Jin-ichi Inokuchi

10. Construction of Sphingolipid Remodeled Cells by Genome Editing

Toshiyuki Yamaji and Yuta Homma

11. Mass Spectrometry of Neutral Glycosphingolipids

Akemi Suzuki, Atit Silsirivanit, Takashi Watanabe, Junko Matsuda, Kei-ichiro Inamori, and Jin-ichi Inokuchi

12. Structural Analyses of the Glycolipids in Lipid Rafts

Yusuke Suzuki and Kazuya Kabayama

13. Extraction, Purification, and Chemical Degradation of LPS from Gut Microbiota Strains

Molly Dorothy Pither, Alba Silipo, Antonio Molinaro, and Flaviana Di Lorenzo

14. NMR Analysis of Mammalian Glycolipids

Yoshiki Yamaguchi

15. Distribution of Glycolipids in the Plasma Membrane Monitored by Specific Probes in Combination with Sodium Dodecyl Sulfate-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

Motohide Murate and Toshihide Kobayashi

16. Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes

Hitoshi Nakayama, Kei Hanafusa, and Kazuhisa Iwabuchi

17. Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes

Kenichi G.N. Suzuki

18. Assays and Utilization of Enzymes Involved in Glycolipid M