Induced Pluripotent Stem Cells and Human Disease: Methods and Protocols

1. Analysis of Mitochondrial Dysfunction by Microplate Reader in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Disorders

Tatiana R. Rosenstock, Congxin Sun, Georgina Wynne Hughes, Katherine Winter, and Sovan Sarkar

2. Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases

Glen Gney-Esken and Fatima Aerts-Kaya

3. Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients

Onur Can Begentas, Dilara Koc, and Erkan Kiris

4. Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias

Xue-Jun Li, Yongchao Mou, Conrad Milton, and Zhenyu Chen

5. Efficient Generation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells for Disease Modeling and Disease Gene Discovery

Satish Kumar, Joanne E. Curran, Sarah Williams-Blangero, and John Blangero

6. Autophagy Dysfunction as a Phenotypic Readout in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Diseases

Congxin Sun, Tatiana R. Rosenstock, Malkiel A. Cohen, and Sovan Sarkar

7. Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Harshini Surendran, and Rajarshi Pal

8. Differentiating Induced Pluripotent Stem Cells toward Mesenchymal Stem/Stromal Cells

Shennea S. McGarvey, Michael Ferreyros, Igor Kogut, and Ganna Bilousova

9. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells

Maryna Pavlova, Shennea S. McGarvey, Ganna Bilousova, and Igor Kogut

10. Image-Based Quantitation of Kainic Acid-Induced Excitotoxicity as a Model of Neurodegeneration in Human iPSC-Derived Neurons

Jana Talbot, Sueanne Chear, Andrew Phipps, Alice Pbay, Alex W. Hewitt, James C. Vickers, Anna E. King, and Anthony L. Cook

11. Amyloid β
(Aβ
) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe, Rei Murakami, and Hideyuki Okano

12. Creating Cell Model 2.0 for Pathogenic Mitochondrial DNA Mutation: iPSC Approach for LHON Mutation

Pragya Singh, Tyler Bahr, Xiaoxu Zhao, Peiqing Hu, Marcel Daadi, TaoSheng Huang, and Yidong Bai

13. CRISPR Guide RNA Library Screens in Human Induced Pluripotent Stem Cells

Yan Zhou, Qiang Fu, Huijun Shi, and Guangqian Zhou

14. Generation and Encapsulation of Human iPSC-Derived Vascular Smooth Muscle Cells for Proangiogenic Therapy

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