Vasculogenic Mimicry: Methods and Protocols

PART I - Vasculogenic Mimicry Background

1. Vasculogenic Mimicry - An Overview

Emily Marques dos Reis and Fernanda Vieira Berti

2. In vitro Models to Study Angiogenesis and Vasculature

Ralph Francescone and Dbora Barbosa Vendramini-Costa

PART II: Protocols for VM in vitro

3. In vitro Tube Formation Assays in Matrigel

Ralph Francescone and Dbora Barbosa Vendramini-Costa

4. Vasculogenic mimicry in a 3D model in vitro

Emily Marques dos Reis, Fernanda Vieira Berti, and Luismar Marques Porto

5. Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry

Mara de Andrade Peixoto, Emily Marques dos Reis, and Luismar Marques Porto

6. A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells

Yarely M. Salinas-Vera, Dolores Gallardo-Rincn, Erika Ruz-Garca, Laurence A. Marchat, Jess Valdz, Carlos Vzquez-Calzada, and Csar Lpez-Camarillo

7. Co-Immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies

Daniel Delgado-Bellido, Angel Garcia-Diaz, and Francisco Javier Oliver

PART III - Protocols for VM In Vivo

8. In vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry

Carlos E. Fonseca-Alves and Patrcia de F. Lainetti

9. Patient derived xenograft models for studying vascular mimicry in melanoma in vivo

Manish Ranjan and Nobel Bhasin

PART IV: VM Analysis

10. Histological evaluation of long-term collagen I culture

Marcella Massimini, Mariarita Romanucci, Raffaella De Maria, and Leonardo Della Salda

11. Histochemical Staining of Vasculogenic Mimicry

Saber Imani, Shuya Liu, Mazaher Maghsoudloo, and QingLian Wen

12. Identification of Vasculogenic Mimicry in Histological Samples

Gustav Stlhammar

13. Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry in vitro

Joseph Manarang and Alison McDermott

14. Immunohistochemistry for VM markers

Alessio Biagioni and Elena Andreucci

15. Confocal laser microscopy for VM analysis with DAPI and phalloidin staining

Karina Cesca and Eliana Medeiros Oliveira

16. Building Mathematical Models for Vascular Growth and Inhibition

Fernanda Vieira Berti and Luismar Marques Porto