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ISBN | 9781071625446 |
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Autor | Israel David |
Vydavatel | Humana Pr |
Jazyk | english |
Väzba | Pevná vazba |
Rok vydania | 2022 |
Počet strán | 226 |
This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries
DNA encoding of chemical structure
design, preparation and analysis of target proteins and tool compounds
screening of soluble protein targets by affinity selection
DEL qPCR, preparative PCR and DNA sequence analysis
computational methods used to analyze selections and choose compounds for resynthesis
and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.